Advanced BioImaging and BioOptics Experimental (AbbE) Platform at Champalimaud Research

Gulbenkian Ciência   Advanced Imaging at IGC

   Bacterial Imaging Cluster at ITQB-NOVA

    Bioimaging Unit at iMM

          Microscopy Facility at NMS


At the COLife bioimaging facilities, researchers can find state-of-the art microscopy equipment and services. Researchers can perform imaging across all dimension scales from nano to mesoscopy.


Bioimaging Techniques @ COLife:

  • Laser scanning confocal microscopy (LSCM / CLSM)
  • Spinning disc confocal microscopy systems (SDCM)
  • Deconvolution widefield microscopy (DWM)
  • Multiphoton microscopy systems (MMS)
  • Total internal reflection fluorescence microscopy (TIRF)
  • Photo activated localization microscopy (PALM)
  • Stochastic optical reconstruction microscopy (STORM)
  • Structured Illumination Microscopy (SIM)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Fluorescence-lifetime imaging microscopy (FLIM)
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence resonance energy transfer (FRET)
  • High-throughput microscopy (HTM)
  • Selective plane illumination microscopy (SPIM)
  • Optical projection tomography (OPT)


Bioimaging Equipment @ COLife:



Bioimaging Training @ COLife:

Optical microscopy at COLife & Friends: learning the basics

The “Optical microscopy at COLife and Friends - learning the basics” workshop covered the optical microscopy techniques and expertise available across COLife.

COLife community webinar: Bioimaging @COLife

In this COLife webinar you will get to know: - the advantages of COLife for the Bioimaging facilities; - future COLife Bioimaging related courses and events; - unique expertise and equipment at each COLife Bioimaging facility; - overview of the size/capacity and diversity of the equipment of all COLife Bioimaging facilities

Introduction to image analysis - COLife and FCUL course

This course aims at providing researchers who are initiating microscopy-based projects with fundamental skills on digital imaging and biological image analysis. The course incorporates theoretical lectures as well as hands-on exercises which demonstrate image analysis procedures on Fiji/ImageJ, the reference open source image analysis software. Finally, trainees will learn about recording and automating image processing workflows. Trainees will be exposed to images produced by transmission, epifluorescence and confocalmicroscopes.


Updated on 22 september 2022

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